Evaluation of sodium dichloroisocyanurate treatment on recovered concentrations of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes from cattle hide surfaces and culture medium

Abstract

AbstractA major concern of the cattle industry is cross‐contamination of meat with pathogens. Cattle are exposed to fecal material, mud, and other contaminants which harbor pathogens that can be shed onto meat and meat processing equipment. Due to increased chances of meat contamination during processing, new antimicrobial formulations for carcass washing before hide removal needs to be identified and tested. Sodium dichloroisocyanurate (SDIC) has biocidal properties and belongs to the N‐halamine group of compounds. Disk diffusion assays revealed, 1,000 ppm SDIC effectively reduced pathogen concentrations. SDIC was evaluated for its effects on pathogens in Tryptic Soy Broth and results revealed that 1,000 ppm SDIC had a strong correlation with time and treatment with no bacterial growth in log CFU ml−1 observed at the lowest detection level. Treatment of inoculated hides with 1,000 ppm SDIC for 5 min resulted in reduction of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes at 1.97, 2.02, and 2.84 log CFU cm−2, respectively.