Abstract
In this study, the yeast strain P10 which was identified to be a member of Aureobasidium pullulans var. melanogenum isolated from the mangrove ecosystems was found to be able to accumulate high content of oil in its cells. After optimization of the medium for lipid production and cell growth by the yeast strain P10, it was found that 8.0g of glucose per 100ml, 0.02g of yeast extract per 100ml, 0.02g of ammonium sulfate per 100ml, pH 6.0 in the medium were the most suitable for lipid production. During 10-l fermentation, a titer was 66.3g oil per 100g of cell dry weight, cell mass was 1.3g per 100ml, a yield was 0.11g of oil per g of consumed sugar and a productivity was 0.0009g of oil per g of consumed sugar per h within 120h. At the same time, only 0.07g of reducing sugar per 100ml was left in the fermented medium. The compositions of the fatty acids produced were C16:0 (26.7%), C16:1(1.7%), C18:0 (6.1%), C18:1 (44.5%), and C18:2 (21.0%). The biodiesel produced from the extracted lipid could be burnt well.
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