Evaluation of single and joint toxicity of perfluorooctanoic acid and arsenite to earthworm (Eisenia fetida): A multi-biomarker approach

Abstract

Perfluorooctanoic acid (PFOA) and arsenic are ubiquitous environmental contaminants and could co-exist in soil. However, data on their possible combined toxic effects on terrestrial organisms are still lacking. In this study, we exposed earthworm Eisenia fetida to artificial soil spiked with different sub-lethal levels of PFOA, arsenite (As(III)) or their mixture for 28 days. The bioaccumulation and multi-biomarker responses in the earthworms were measured. Results showed that the co-existence of PFOA and As(III) in soil enhanced the bioaccumulation of arsenic while reduced the bioaccumulation of PFOA. Most selected biomarkers exhibited significant responses at higher exposure levels and indicated oxidative damages. Biomarker Response Index (BRI) was used to integrate the multi-biomarker responses and the results showed significant dose-effect relationships between biological health status and exposure levels. Moreover, variation analysis of multi-biomarkers and BRI proved that As(III) exhibited more toxicity than PFOA to the earthworms. Based on BRI results, Effect Addition Index (EAI) was calculated to evaluate the joint effects of the two toxicants. According to EAI, the joint toxicity of PFOA and As(III) was related to exposure concentration, changing from synergism to slight antagonism with the increase of exposure level. These results provide valuable toxicological information for the risk assessment of co-exposure to PFOA and arsenic in the soil environment. Moreover, this study proved that BRI is an effective tool to integrate multi-biomarker responses, and its combination with EAI provides a useful combined approach to evaluate the joint effects of mixed contamination systems.