Evaluation of silica nanoparticle binding to major human blood proteins.

Katsutomo Hata,Yohei Mukai,Kazuma Higashisaka,Shin-Ichi Tsunoda,Yasuo Yoshioka,Yasuo Tsutsumi,Haruhiko Kamada,Kazuya Nagano

doi:10.1186/1556-276x-9-668

Abstract

Nanomaterials are used for various biomedical applications because they are often more effective than conventional materials. Recently, however, it has become clear that the protein corona that forms on the surface of nanomaterials when they make contact with biological fluids, such as blood, influences the pharmacokinetics and biological responses induced by the nanomaterials. Therefore, when evaluating nanomaterial safety and efficacy, it is important to analyze the interaction between nanomaterials and proteins in biological fluids and to evaluate the effects of the protein corona. Here, we evaluated the interaction of silica nanoparticles, a commonly used nanomaterial, with the human blood proteins albumin, transferrin, fibrinogen, and IgG. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that the amount of albumin, transferrin, and IgG binding to the silica particles increased as the particle size decreased under conditions where the silica particle mass remained the same. However, under conditions in which the specific surface area remained constant, there were no differences in the binding of human plasma proteins to the silica particles tested, suggesting that the binding of silica particles with human plasma proteins is dependent on the specific surface area of the silica particles. Furthermore, the amount of albumin, transferrin, and IgG binding to silica nanoparticles with a diameter of 70 nm (nSP70) and a functional amino group was lower than that with unmodified nSP70, although there was no difference in the binding between nSP70 with the surface modification of a carboxyl functional group and nSP70. These results suggest that the characteristics of nanomaterials are important for binding with human blood proteins; this information may contribute to the development of safe and effective nanomaterials.

Highlights

The development and use of materials at the nanometer scale already have multiple applications in various fields, including the food and cosmetics industries; such nanomaterials have become indispensable in our lives [1,2]
Effects of particle size on binding to proteins we evaluated whether the differences in the size of the silica particles affected their binding to albumin, transferrin, fibrinogen, and immunoglobulin G (IgG)
We found that the binding of silica particles to albumin, transferrin, and IgG appears to increase as the particle size decreased (Figure 1A,B,C)

Summary

Introduction

The development and use of materials at the nanometer scale already have multiple applications in various fields, including the food and cosmetics industries; such nanomaterials have become indispensable in our lives [1,2]. Silica nanoparticles have been widely used in many consumer products because of their useful properties, including relatively low production costs, easy separation, and ease of modification of their surface properties. They have attracted the attention of the Nanomaterial-mediated biological effects are related to physical characteristics such as particle size and the surface properties of the nanomaterials. To fully realize the potential of the protein corona, it is essential to understand and quantify the effects of individual proteins on the characteristics of nanomaterials. Analyses of the amount of binding of each individual human plasma protein to nanomaterials with various properties would be invaluable in developing the safety profile of such nanomaterials

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Discussion

Conclusion