Abstract
In Belgium, it is a general recommendation to collect swabs and rinses in rape cases. We evaluated the need of both prelevations for successful DNA analysis based on historical data. DNA concentrations and success rate of STR analysis of a total of 213 PSA positive rinses (including 29 anal and 184 vaginal rinses) and 256 PSA positive swabs (including 55 anal and 201 vaginal swabs) were compared. The study includes 154 cases where both a swab and a rinse were analysed simultaneously. Considering all vaginal and anal swabs and all rinses together, the median of the DNA concentration of DNA isolates of swabs is approximately 2.5 times higher than those of the DNA isolates of rinses. Moreover the success rate of obtaining an autosomal STR profile useful to identify the assailant is 72% for swabs versus 65% for rinses for the cases where both a swab and a rinse were examined simultaneously. In conclusion, swabs were found to have a higher DNA yield and a higher success rate of STR analysis for identifying the alleged assailant than rinses.
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