Evaluation of serum Hepcidin level and Iron profile among Sudanese patients with anemia of end stage kidney disease

Abstract

ackground: Anemia of chronic disease is anemia found in certain chronic disease state, is typically marked by the disturbance of iron homeostasis or hypoferremia. This condition leads to shortage of iron for hemoglobin synthesis but the iron storage in bone morrow is left undisturbed. Patients with chronic kidney disease are usually anemic because of defective erythropoeisis and inflammation. Materials and methods: Some of red blood cell profile (Hb, PCV, RBCs count and RBCs indices) were determined by the automated Hematology Analyzer and Cobas e 411 was used to determine the levels of serum iron, ferritin, TIBC, and transferrin saturation percentage. Enzyme – Linked immunoassay (ELISA) was used to determine the level of hepcidin. Results: The results show the mean of the RBCS profile (RBCs count, Hb, PCV) (3.353±88cell/l, 10.62±2.4g/dl, 32.59±6.82%) in patients with ACKD Vs (4.048±0.47cell/l, 12.52±1.57g/dl, 37.92±4.79%) in control groups P.value (0.000, 0.000, and 0.000) respectively. Serum hepcidin levels higher in patients with ACKD compared with healthy controls mean (161.55±29.8ng/ml Vs 82.05±13.4ng/ml. P. value (0.000). The mean value of the iron profile, S. iron, S. ferritin and TS % (61.353±29,8ug/dl, 195.3.62±19.4ng/ml, 21.59±12.82%) in patients with ACKD Vs (82.048±0.47ug/dl, 80.52±1.57ng/ml, 28.92±4.79%) in control groups P.value (0.000, 0.000, and 0.000) respectively. Conclusion: In the present study there is significant association between CKD and RBCS profile (RBCs count, Hb, PCV). The hepcidin levels were significantly higher in patients with ACKD compared with healthy controls. The Statistical significant differences showed in the comparison between the study variables (RBCs profile, Iron profile, hepcidin level) and the end stage of CKD (dialysis dependent), in the RBCs count, Hb, PCV, S. iron, S.ferritin, TIBC. TS %, hepcidin level.