Abstract
The aim of this study was to investigate the effect of grilling and brining on the sensory properties, the fillet fatty acid composition and the cardio-protective activity of sardine (Sardina pilchardus), studying the in vitro activity against Platelet-Activating-Factor (PAF) induced platelet aggregation. Sensory evaluation of grilled and brined sardine showed that grilled sardine had higher scores for the attributes: grilled fish, marine and fresh fish whereas brined sardine had higher scores for the attributes: salty, iodine, oily and bitter. Grilled sardine exhibited significantly increased fillet fatty acid content while the brined fish sample significantly decreased fatty acid levels. Polar lipids of all specimens (raw, grilled and brined) showed strong inhibitory activity against PAF action indicating that grilling and brining have not diminished the cardio-protective properties of sardine.
Highlights
The benefits of a diet rich in fish on human health have been linked to ω-3 fatty acids’ content, especially eicosapentaenoic acid (EPA; 20:5ω-3) and docosahexaenoic acid (DHA; 22:6ω-3) [1]
The aim of the current study was to investigate the effect of grilling and brining of sardine (Sardina pilchardus) on the sensory properties, the fish fillet fatty acid composition and the in vitro biological activities of fish polar lipids to induce platelet aggregation or/and inhibit the PAF-induced platelet aggregation
Grilled and brined sardine samples exhibited statistically elevated Total lipids (TL) levels in comparison to raw sardine TL levels (Table 2), data that is in accordance with the literature [21,22]
Summary
The benefits of a diet rich in fish on human health have been linked to ω-3 fatty acids’ content, especially eicosapentaenoic acid (EPA; 20:5ω-3) and docosahexaenoic acid (DHA; 22:6ω-3) [1]. These fatty acids are reputed to have beneficial properties against cardiovascular diseases (CVDs) [2] by lowering blood pressure and serum triglycerides and improving endothelial function and plaque stability [3]. Atherosclerosis is a chronic inflammatory condition with clinical end points of heart attack and stroke. PlateletActivating-Factor (PAF) [6] is a potent inflammatory
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