Abstract
Three beach soils in Lagos, Nigeria were screened for the presence of antibiotic producing fungi against 8 test pathogenic bacteria & fungi. The physiochemical parameters of the soils were determined following standard procedures. Soil plate dilution method was employed for isolation of marine fungi and they were identified based on cultural and microscopic characteristics. Primary screening of isolated fungi for antibiotic potential was determined by perpendicular streak method against known pathogenic test organisms (Escherichia coli, Saphylococcus aureus, Klebsiella pnuemoniae, Pseudomonas aeruginosa, Candida albicans, Saccharomyces cerevisiae,Aspergillus niger and Aspergillus fumigatus). Further sreening of the product of secondary metabolism [antibiotic] of the active fungi was done by agar disc diffusion assay. The fungus with the highest secondary activity was subjected to molecular analysis for further identification. pH of beach soils was alkaline ranging from 7.6-8.0, while temperature and moisture were normal ranging from 26.333 ± 1.155a-30.333 ± 0.577aand 7.142 ± 1.497a-10.030 ± 3.130a respectively. A total of four fungal species of 2 different genera were isolated from the beaches ;Aspergillus niger,Aspergillus flavus,Penicillium purpurogenum and Penicillium islandicum. Both primary and secondary assays revealed only antibacterial activities against Staphylococcus aureus and Psedomonas aeruginosa with no effect on all test fungi. Highest antibacterial activity[28+0.2a] was exhibited by the fungus identified as Penicillium purpurogenum. Fungi from beaches in Lagos, Nigeria may be a promising source of antibacterial agent useful in the treatment of infections caused by S. aureus and P. aeruginosa.
Highlights
Soils are traditionally the main source of fungi [1] and other numerous microorganisms producing structurally complex bioactive products of pharmaceutical importance
The marine and fresh water environments are known to contain taxonomically diverse fungi groups which exhibit unique physiological and structural characteristics that enable them to survive in extreme environmental conditions, with the potential production of novel secondary metabolites not observed in terrestrial microorganisms
The test organisms used for this study were; Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pnuemoniae, Candida albicans, Aspergillus fumigatus, Aspergillus niger and Saccharomyces cerevisiae obtained from the culture collection unit of Department of Microbiology, University of Ilorin, Nigeria and University of Ilorin Teaching Hospital, Ilorin, Nigeria
Summary
Soils are traditionally the main source of fungi [1] and other numerous microorganisms producing structurally complex bioactive products of pharmaceutical importance. The constant emergence of multidrug resistant pathogens has led to substantial morbidity and mortality and to overcome this, it is necessary to discover novel antibiotics by continuous screening of secondary metabolites from microbial sources This present study is designed with a view to isolate, screen and characterize antibiotic producing fungi from marine and fresh water soils in the cities of Lagos and Ilorin, Nigeria. The test organisms used for this study were; Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pnuemoniae, Candida albicans, Aspergillus fumigatus, Aspergillus niger and Saccharomyces cerevisiae obtained from the culture collection unit of Department of Microbiology, University of Ilorin, Nigeria and University of Ilorin Teaching Hospital, Ilorin, Nigeria They were confirmed using standard methods [9] and routinely subcultured for purity. Filtration was carried out twice to obtain cell-free supernatant antibiotic agent for antibacterial activity [11]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
