Evaluation of sea mustard (Undaria pinnatifida) sporophylls from South Korea as fucoidan source and its corresponding antioxidant activities

Therese Ariane N Neri,Zuliyati Rohmah,Byeong-Dae Choi,Bernadeth F Ticar,Grace N Palmos

doi:10.1186/s41240-019-0141-4

Abstract

Sporophylls from sea mustard, Undaria pinnatifida, which are by-products in seaweed production industries, were taken from Hansan Island, Tongyeong, and Gijang, Busan, and investigated for their fucoidan content and corresponding antioxidant activities. The extracted fucoidan yield from sporophylls of sea mustard samples from Tongyeong (TF) and Gijang (GF) were 12.1% and 13.6%, respectively. The antioxidant activities assessment of TF and GF extracts showed relative significant difference between samples, suggesting effect of location on bioactivities of fucoidan samples. GF was 50–68% more effective than TF against DPPH and superoxide radicals samples which may be attributed to the difference in their degree of sulfation and monosaccharide composition. Meanwhile, TF exhibited greater scavenging activity against hydroxyl radicals compared to GF which could be due to protein impurities.

Highlights

With the advent of health awareness and health-conscious buyers, consumption of seaweeds and related goods has progressively increased in the market
Chemical and radical reagents including 1,1-diphenyl-2picrylhydrazyl (DPPH), nicotinamide adenine dinucleotide (NADH), nitro blue tetrazolium (NBT), phenazine methosulfate (PMS), ethylenediaminetetraacetic acid (EDTA), 1, 9-dimethylmethylene blue (DMMB), 3-methyl-2-benzothiazolinone hydrazine hydrochloride (MBTH), and hydrogen peroxide (H2O2) as well as fucoidan from Fucus vesiculosus, D-galactose, bovine serum albumin (BSA), Lfucose, chondroitin-6-sulfate, N-acetyl-D-glucosamine, and D-glucuronic acid standards were all purchased from Sigma-Aldrich
Fucoidan extracted from sea mustard sporophylls from Tongyeong (TF) and Gijang City (GF) both demonstrated lower scavenging activity on DPPH, superoxide, and hydroxyl radicals when compared with ascorbic acid as positive control and pure fucoidan standard as sample control

Summary

Introduction

With the advent of health awareness and health-conscious buyers, consumption of seaweeds and related goods has progressively increased in the market. Seaweeds are nutrient-rich food source mainly composed of proteins, amino acids, non-protein nitrogen, lipids, dietary fiber, vitamins, and minerals, as well as various bioactive compounds which are significant for biomedical, pharmaceutical, and health products. Bioactive compounds derived from seaweeds have been studied for anti-inflammatory, antiviral, anti-thrombic, and anticoagulant activities, anticancer and antitumor properties, anti-obesity, anti-diabetic, antihypertensive, and anti-hyperlipidemic capabilities (Bedoux et al 2014; Smit 2004). From the family of Reactive oxygen species (ROS), which include superoxide (O2−) and hydroxyl radicals (OH), and hydrogen peroxides (H2O2) are considered double-edged swords. Oxidative stress takes a major part in the progression of some ailments like atherosclerosis, hypertension, aging, Alzheimer’s disease, kidney malfunction, and even cancer (Roberts and Sindhu 2009), and accumulated evidence supported the relationship of increased oxidative stress with

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