Evaluation of Rice Resistance to Southern Rice Black-Streaked Dwarf Virus and Rice Ragged Stunt Virus through Combined Field Tests, Quantitative Real-Time PCR, and Proteome Analysis.

Zhenchao Wang,Qi Zhao,Zhongcheng Yang,Linhong Jin,Yongtian Wei,Longlu Ran,Enlong Liu,Xiangyang Li,Lu Yu,Deyu Hu,Zhuo Chen,Baoan Song,Wenli Wang,Rong Guo

doi:10.3390/v9020037

Zhenchao Wang, Qi Zhao + Show 12 more

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https://doi.org/10.3390/v9020037

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Journal: Viruses	Publication Date: Feb 22, 2017
Citations: 10	License type: CC BY 4.0

Affiliation: Guizhou University, Institute of Plant Protection

Abstract

Diseases caused by southern rice black-streaked dwarf virus (SRBSDV) and rice ragged stunt virus (RRSV) considerably decrease grain yield. Therefore, determining rice cultivars with high resistance to SRBSDV and RRSV is necessary. In this study, rice cultivars with high resistance to SRBSDV and RRSV were evaluated through field trials in Shidian and Mangshi county, Yunnan province, China. SYBR Green I-based quantitative real-time polymerase chain reaction (qRT-PCR) analysis was used to quantitatively detect virus gene expression levels in different rice varieties. The following parameters were applied to evaluate rice resistance: acre yield (A.Y.), incidence of infected plants (I.I.P.), virus load (V.L.), disease index (D.I.), and insect quantity (I.Q.) per 100 clusters. Zhongzheyou1 (Z1) and Liangyou2186 (L2186) were considered the most suitable varieties with integrated higher A.Y., lower I.I.P., V.L., D.I. and I.Q. features. In order to investigate the mechanism of rice resistance, comparative label-free shotgun liquid chromatography tandem-mass spectrometry (LC-MS/MS) proteomic approaches were applied to comprehensively describe the proteomics of rice varieties’ SRBSDV tolerance. Systemic acquired resistance (SAR)-related proteins in Z1 and L2186 may result in the superior resistance of these varieties compared with Fengyouxiangzhan (FYXZ).

Highlights

Rice is often subjected to biotic stressors, such as fungi, bacteria, and viruses [1]
In early July of 2012, rice varieties were cultivated in Jiucheng during the explosion of southern rice black-streaked dwarf virus (SRBSDV) and rice ragged stunt virus (RRSV)
The yield test showed that 22 candidates were categorized into four classes, in which H658 (A.Y., 2085.05 kg) and Z1 (A.Y., 2023.74 kg) with the highest harvest yields included in Group 1, and YX1, S5, C3727, M19, Y1 with the lowest harvest yields were named as Group 4, the A.Y. of which range from 183.92 to 784.85 kg

Summary

Introduction

Rice is often subjected to biotic stressors, such as fungi, bacteria, and viruses [1]. Rice production in China has suffered from two emerging viruses, namely, southern rice black-streaked dwarf virus (SRBSDV) and rice ragged stunt virus (RRSV) [2,3]. Viruses 2017, 9, 37 family Reoviridae [4,5] and possesses a genome consisting of 10 double-stranded RNA (dsRNA); this virus is prevalent in Southern Asian countries, Vietnam, Japan, and China [6,7,8,9]. RRSV causes diseases with symptoms of twisted leaves, delayed flowering, nodal branch production, incomplete panicle emergence, and panicles bearing mostly unfilled grains; this virus is prevalent in China, Philippines, and Vietnam [14,15,16,17]. The epidemic and outbreak of diseases caused by SRBSDV and RRSV are closely associated with the outbreak of viruliferous populations of BPHs and WBPHs [21,22,23,24]

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