Abstract
Actinobacteria were isolated from rhizosphere soil and its plant growth-promoting ability was tested. Actinobacteria were screened for promoting plant growth by phosphate and zinc solubilization, gibberellin, siderophore, HCN and indole-3-acetic acid production. The abiotic stresses of all the isolates were tested for parameters such as pH, temperature and NaCl. Upon screening, a potent actinobacteria named Streptomyces sp. ZS-18 was isolated and identified based on the colony characteristics. The potent strain solubilized both the zinc oxide (26 ± 0.75mm) and zinc carbonate (22 ± 0.88mm). The utilization of gibberellic acid (80μg/ml) by the potential strain ZS-18 showed maximum production measured by optical density value of 0.179. The growth of the green gram plant was determined by shoot, root, total length, number of leaves and chlorophyll estimation of the leaves. Streptomyces sp. ZS-18 showed good growth on ISP 1, ISP 2, ISP 3, ISP 5 and ISP 7 medium and moderate growth was observed in ISP 4 and ISP 6 medium. In addition, Actinobacteria were tested for antagonistic activity against plant pathogens. The isolate ZS-18 in the pot trial experiment showed increased growth in comparison with control and cow dung manure.
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