Evaluation of renoprotective and Anti-inflammatory effects of doxycycline in carrageenan-induced renal inflammation

Abstract

The present study was designed to investigate biochemical changes induced by doxycycline treatment of carrageenan-induced renal inflammation. For this purpose, 60 rats divided into four groups. Group (1) used as a control and administrated distilled water (1 ml/kg, i.p.) daily for 5 days. Group (2) carrageenan group: rats administrated 2% λ- Carrageenan (25 µl/kg, i.p.) as one dose at first day of experiment. Group (3) doxycycline rats were received doxycycline (100 mg/kg, i.p./day/5 days). Group (4) carrageenan+ doxycycline rats were received doxycycline (100 mg/kg, i.p.), after an hour 2% λ- carrageenan (25 µl/kg, i.p.) as one dose at first day of experiment. The experiment was continued for 5 days. Collection of samples was performed on 1st, 3rd and 5th day of experiment. Serum was used for evaluation of kidney function (urea, creatinine, Na+ and K+) and plasma proteins (total protein, albumin, and globulin), as well as inflammatory markers and cytokines (TNF-α, IL-6, CRP, and NO). Kidney tissue used for estimation of antioxidant parameters (MDA and GPx), and histopathology. The results revealed that there were significant increases in urea, creatinine, MDA, TNF-α, IL-6, CRP, and NO in carrageenan group, while GPx, Na+ and K+ showed significant decreases. Moreover, histological examination of group 2 showed degenerative changes in the lining epithelium of renal tubules associated with peritubular mononuclear cells infiltration compared with control group. Therefore, it could be concluded that doxycycline has anti-inflammatory role.