Abstract
Objective: The main objective of periodontal therapy is tissue regeneration. Previous studies have identified the potential of mesenchymal stem cells to improve major periodontal defect reconstruction in bone tissue engineering. Cell sheet technology (CST), in which a cell culture is obtained from a material coated with a temperature-sensitive substrate, has been developed for the reconstruction of various tissues, including periodontal tissue. Cementum protein-1 (CEMP-1) is a 50-kDa protein that plays a crucial role in cementogenesis by enhancing the combining of cells formed by cell cementoblast. Evaluate periodontal tissue regeneration with CST, and the application of chitosan, chitosan cell sheet or arginineglycineaspartic acid (RGD)-modified chitosan cell sheet on Macaca nemestrina one-wall defect model.Methods: The CEMP-1 was analyzed expression in a sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel electrophoresis assay.Results: CEMP-1 expression in gingival crevicular fluid was observed using the SDS-PAGE method every week for 3 weeks. Protein band expressions on SDS-PAGE gel were identified at around 50 kDa with different thicknesses between groups. The chitosan, chitosan cell sheet, and RGD-modified chitosan cell sheet groups showed protein bands of CEMP-1 between 50 and 70 kDa at weeks 1, 2, and 3; weeks 2 and 3; and weeks 1 and 2, respectively.Conclusion: Our results demonstrated that the application of chitosan and RGD-modified chitosan cell sheets could enhance bone regeneration, as evidenced by CEMP-1 protein expression levels.
Highlights
Periodontitis is a chronic inflammatory disorder that degrades the integrity of dental support tissues, which include alveolar bones, periodontal ligament, cementum, and gingiva [1]
Our results demonstrated that the application of chitosan and RGD-modified chitosan cell sheets could enhance bone regeneration, as evidenced by Cementum protein-1 (CEMP-1) protein expression levels
In week 2, the protein band was seen in the chitosan group and RGDmodified chitosan cell sheet group, and it started to be seen as a thin layer in the chitosan cell sheet group
Summary
Periodontitis is a chronic inflammatory disorder that degrades the integrity of dental support tissues, which include alveolar bones, periodontal ligament, cementum, and gingiva [1]. Periodontal regeneration therapy is an alternative therapy for periodontal disease that restores tissue to its previous form and function This technique aims to eliminate granulation and necrotic tissue at the bone defect area and induce healing. A one-wall bone defect or horizontal bone defect has less healthy cells and a large defect, causing difficulties in the ability of grafts to produce regenerative cells that are similar to or that resemble the lost tissue [4,5]. To overcome this limitation, several combinations of regenerative therapies and tissue engineering applications have been studied
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
