Abstract
Histologically verified pairs (n= 10) of pancreatic tumors and non-neoplastic tissues were used for quantitative real-time PCR and the stability of 24 reference genes was analyzed with geNorm and NormFinder software. Raw Cqvalues correlated with the degree of RNA degradation. This correlation was abolished by normalization to Cqof 18S endogenous control gene. Both geNorm and NormFinder programs suggested EIF2B1, ELF1, MRPL19, and POP4 as the same most stable genes. We have thus identified suitable reference genes for future expression studies in pancreatic carcinoma. Normalization method reducing the effects of RNA degradation on the quality of results was also developed.
Highlights
Pancreatic cancer is the fourth leading cause of cancer death in the United States [1] as well as in the Czech Republic [2]
The total RNA was isolated from surgically removed tumor and non-neoplastic tissues of 10 patients with pancreatic carcinoma diagnosis
Research of pancreatic cancer is hindered by difficulties with the recruitment of pancreatic cases with brief survival and poor performance status
Summary
Pancreatic cancer is the fourth leading cause of cancer death in the United States [1] as well as in the Czech Republic [2] It has a dismal five-year survival less than 5% [3], primarily related to the fact that diseasespecific symptoms occur late in the course of the disease. A number of methods have been developed to study gene expression. Pancreatic tumors have low neoplastic cellularity and a predominance of nonneoplastic fibrous (or desmoplastic) stroma. This is rather unique to duct adenocarcinomas of the pancreas; in contrast to infiltrating carcinomas arising in other organs.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
