Evaluation of quality of Salvia miltiorrhiza Bunge from different provenances by HPLC-DAD fingerprint combined with Chemometrics Method

Abstract

Objective To compare the difference of HPLC fingerprints of Salvia miltiorrhiza Bunge from different seed sources under the same ecological environment and harvest time, combined with chemometrics. Methods A DiamonsilC18 (250 × 4.6 mm, 5 μ m)column was used with a 0.02% phosphoric acid-water (methanol: acetonitrile = 1:1) elution system. The detection wavelength was 270 nm, the flow rate was 1 ml / min and the column temperature was 30 °C. The HPLC fingerprints of Salvia miltiorrhiza Bunge from 9 different provenances were tested, and the similarity, cluster analysis and principal component analysis (PCA) of relative peak area were carried out. Results The HPLC fingerprints of Salvia miltiorrhiza Bunge from different provenances were established, and 27 common peaks were established, with the similarity of 0.989 and 0.999. All sample were divided into 3 groups by systematic cluster analysis, and the PCA score map and payload map were obtained by principal component analysis (PCA). The main chemical components which lead to the difference were selected by VIP value as peak 19, peak 18, peak 17 and peak 2. Conclusion There was no significant difference in the composition of Salvia miltiorrhiza Bunge, but there was a great difference in the content of components.