Abstract
1. Metabolomics of Four Biofluids from Dairy Cows: Potential Biomarkers for Milk Production and Quality Hui-Zeng Sun, Di-Ming Wang, Bing Wang, Jia-Kun Wang, Hong-Yun Liu, Le Luo Guan, Jian-Xin Liu Journal of Proteome Research CrossRef
Highlights
The methods generally used for determining rumen microbial protein synthesis depend on the use of natural microbial markers such as RNA and DAPA or isotopes of 35S, 15N and 32P
A number of studies in ruminant species (Daniels et al, 1994; Chen et al, 1995) indicated that purine metabolites: creatinine (PMC) index can predict microbial nitrogen (MN) supply with reasonable accuracy as it is little affected by sampling time, while the data of Shingfield and Offer (1998) indicated a diurnal variation in the PMC index
This study examined the influence of levels of feed intake on concentration of PM in urinary spot samples and evaluated the use of PMC in spot urine samples as an on farm diagnostic tool to estimate the MN supply in goats
Summary
The methods generally used for determining rumen microbial protein synthesis depend on the use of natural microbial markers such as RNA (ribonucleic acid) and DAPA (diaminopimelic acid) or isotopes of 35S, 15N and 32P. The prediction of microbial protein using urinary PM has special interest since it overcomes the disadvantages of other conventional methods. This technique is non invasive and simple by avoiding the need for fistulated animals and those measurements related to digesta and microbial marker kinetics. A logical step forward would be to use the concentration of PM in spot urine samples as an index of microbial protein supply. This study examined the influence of levels of feed intake on concentration of PM in urinary spot samples and evaluated the use of PMC in spot urine samples as an on farm diagnostic tool to estimate the MN supply in goats
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