Evaluation of Protective Effects of Polyphenols of the Marine Brown Alga Ecklonia cava against Potassium Bromate Induced Nephrotoxicity in Rats

Abstract

Pharmacognosy Research,2023,15,3,544-550.DOI:10.5530/pres.15.3.057Published:June 2023Type:Original Article Authors:Moqbel Ali Moqbel Redhwan, Suman Samaddar, Hariprasad MG, Sumaia Abdulbari Ahmed Ali Hard, Gitima Deka, and Anyembe Godfrey Author(s) affiliations:Moqbel Ali Moqbel Redhwan1,*, Suman Samaddar2, Hariprasad MG3, Sumaia Abdulbari Ahmed Ali Hard4, Gitima Deka5, Anyembe Godfrey6 1Department of Pharmacology, KLE College of Pharmacy, Bangalore, Karnataka, INDIA. 2BGS GIMS Research Institute, BGS Global Institute of Medical Sciences, Bengaluru, Karnataka, INDIA. 3Department of Pharmacology, KLE College of Pharmacy, Bangalore, Karnataka, INDIA. 4Department of Pharmaceutics, KLE College of Pharmacy, Bangalore, Karnataka, INDIA. 5Department of Pharmacy, Yeungnam University, Gyeongsangbuk-do, SOUTH KOREA. 6Department of Pharmacology, Karnataka College of Pharmacy, Bangalore, Karnataka, INDIA. Abstract:Background: Ecklonia cava is a kelp (brown algae) genus belonging to Lessoniaceae with plenty of Eckol-type phlorotannins. It exhibits antioxidant, anti-inflammatory, and antibacterial activity. Objectives: This study investigated Ecklonia cava (EC) polyphenols for their protective effects against KBrO3-induced nephrotoxicity in rats. Materials and Methods: The polyphenolic fraction was isolated from EC. Group, I was control (untreated), and group II was administered KBrO3 (135 mg/kg b.w) intragastric for four weeks. Group III was administered ECPP (200 mg/kg b.w) concurrently with KBrO3 (135 mg/kg b.w) orally, and Group VI was administered Rutin (100 mg/ kg b.w) along with KBrO3 (135 mg/kg b.w) orally. The protective effects of ECPP on KBrO3-induced nephrotoxicity in rats were assessed for the biochemical parameters of serum, various antioxidant enzymes, and histopathological changes in kidneys. Results: The level of serum of Blood Urea Nitrogen (BUN), uric acid, and creatinine was suggestively augmented (p<0.001) when treated by KBrO3. The activity of antioxidant enzymes, superoxide dismutase, catalase, glutathione peroxidase, and FRAP (ferric ion reducing antioxidant parameter) were diminished (p<0.001). At the same time, lipid peroxidation and nitric oxide were raised (p<0.001) with KBrO3 treatment in the kidneys. In addition, the protein carbonyl level was amplified (p<0.001) with KBrO3 administration. Histological studies presented renal damage in KBrO3-treated animals where tissue injury was abridged in ECPP pretreatment groups. Conclusion: These results suggest that ECPP functions as an antioxidant in vivo by scavenging reactive oxygen species, which helps preclude oxidative renal injury in rats treated with KBrO3. Keywords:Antioxidant, Ecklonia cava, Nephroproductive activity, Polyphenols, Potassium bromate.View:PDF (1.04 MB)