Evaluation of prophylactic activity of drugs on swine gastroesophageal ulcer induced by betazole-reserpine using the method of endoscopy.

Abstract

Prophylactic activity of pepstatin (PEP), alminium silicate (Al-Si), sodium polyacrylate (PAS) and sodium alginate (AGS) on the experimentally produced gastroesophageal (GE) ulcer of swine was evaluated by the method of serial endoscopic observation. A total of 23 pigs which could be observed little pathological change on GE area out of 28 pigs with gastric cannulae was used in the experiments. The GE uicer was produced in pigs which were subcutaneously injected betazole (50 mg/body) and reserpine (0.02mg/kg) once a day for 3 days (6/6). The pig was fed a small amount of mashed feed during test period. The result from the same experiment performed on the pig which had a test substance with mashed feed revealed that the administration of 20 mg/day of PEP and 20 g/day of PAS had an effective prophylactic activity on GE ulcer. On the other hand, the addition of Al-Si or AGS to mashed feed did not protect the GE area from ulceration. In a separate experiment, acidity and peptic activity of stomach content were determined 1 hr after ingestion of the feed. The determination of acidity and peptic activity revealed a positive antacidic and antipeptic activity in Al-Si and PEP treated animals, repectively. The prophylactic activity of PEP may be due to its antipeptic activity. Since PAS showed neither antacidic nor antipeptic activity, the mechanism of its antiulcerogenic activity remains to be elucidated.