Abstract
ABSTRACTThis work studied the resistance of Saccharomyces cerevisiae Y904 to ethanol on an alcoholic fermentation process operated in fed-batch. The effect of temperature, inoculum size and substrate concentration on fermentation yield, productivity and residual sugars concentration was studied by a central composite design (CCD). Based on the CCD study, it was determined the optimum values of 240, 35 g/L, and 26°C for total reducing sugars, inoculum concentration and temperature, respectively. This set of conditions experimentally enabled a productivity of 6.0 g/L h, a yield of 93% and an alcohol content of 113.6 g/L, after 10 h of fermentation. When yeast cells were adapted at 4°C, the inoculum pH adjusted to 2.5 and sugarcane broth used as substrate, a 94% yield and a 10.1 g/L. h productivity were achieved.
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