Abstract
The development of non-antibiotic and environmentally friendly agents is a key consideration for health management in shrimp aquaculture. In this study, the probiotic potential in shrimp aquaculture of Pediococcus pentosaceus MR001, isolated from Macrobrachium rosenbergii, was investigated by means of feeding trial and genetic characterization. In the feeding trial, dietary supplementation with P. pentosaceus MR001 significantly increased weight gain and digestive enzyme activity (p < 0.05) in shrimp, Litopenaeus vannamei. The intestinal histology showed that shrimp given the probiotic diet had healthier guts than the control group. Also, the immune gene expression and the survival rate in the treatment group were significantly increased when compared with the control group. The genetic characteristics of P. pentosaceus strain MR001 were explored by performing whole-genome sequencing (WGS) using the HiSeq 2500 platform and PacBio system, revealing the complete circular genome of 1,804,896 bp. We also identified 1789 coding genes and subsequently characterized genes related to the biosynthesis of bacteriocins, stress resistance, and bile tolerance. Our findings suggest that insights in the functional and genetic characteristics of P. pentosaceus strain MR001 could provide opportunities for applications of such strain in shrimp diet supplementation.
Highlights
Epidemics are increasingly recognized in many countries as one of the most important constraints on cultivated shrimp production
Twelve lactic acid bacteria (LAB) were isolated from giant freshwater prawn gut and showed antagonistic ability against V. harveyi
The results suggest that P. pentosaceus MR001 might effective at protecting cells against oxidative stress
Summary
Epidemics are increasingly recognized in many countries as one of the most important constraints on cultivated shrimp production. Probiotics are live microorganisms that play an important role in the microbial balance of the host organisms. They can reduce pathogenic microbes, stimulate growth rate, and improve animal health[1]. Studies have shown that P. pentosaceus can enhance innate immunity, physiological health and resistance to pathogens in fish[4] and shrimp[9,10]. Despite these findings, little is known about its genetic information and functions, compared with another closely related species P. acidilactici, which is widely used in aquaculture[11,12]. NaCl Bile salt was evaluated, and the whole genome was characterized in order to further evaluate the potential of this strain as a probiotic dietary supplement in marine aquaculture
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