Evaluation of Potential Native Phosphate Solubilizing Bacteria for the Agriculture Practice of okra (Abelmoschus Esculentus) with the Target to Replace Chemical Fertilizer - in a Field Study

T Ketharin,V Jaamuna,P T Mohamed Javad,P Paulraj,M Chandramohan,P Sajeesh,N Jayakumar,K P Sajna

doi:10.14419/ijet.v7i4.14.27468

Abstract

Phosphorus (P) is a major growth limiting nutrient. Phosphate Solubilizing Bacteria (PSB) plays an important role in the development of agricultural industry. In this study, the native PSB were isolated from the okra plant root by using Pikovskaya’s medium and potential strains were selected based on the phosphate solubilization efficiency. The potential strains in presence of Rock Phosphate (RP), Tea dust, Eggshells and Bone waste of animal (TEB) were studied as an alternative fertilizer instead of chemical fertilizer. The PSB consortia strains were prepared and inoculated to the plant root. Different growth parameters were studied to determine the effectiveness of strains. In all cases the difference was significant with p<0.05. A decreasing trend of results were recorded among the tested parameters from plants in PSB with RP, PSB with TEB, and PSB with TSP and followed by PSB without fertilizer. The highest results were recorded among plants in PSB with RP for mean shoot length (64cm), mean weight of fruits (29gm), mean length of fruits (23cm), mean number of leaves (13Nos) and chlorophyll content (5.1mg/g). Hence, the present study showed that the application of PSB with the cheap source of nutrients has shown the capability of providing similar or enhanced results based on the various characters expressed by the plant and thus reduce the reliance on synthetic chemical fertilizers.

Highlights

Okra (Abelmoschus esculentus) is an essential vegetable crop which supplies greater nutrition consists of protein, fats, carbohydrates, vitamins and minerals [1]
Root colonization by potential Phosphate Solubilizing Bacteria (PSB) tend to improve existing root growth length or by auxin hormone production from indole-3acetic-acid by the plant [15]. This present study was aimed to isolate potential phosphate solubilizing bacteria (PSB) from rhizosphere and non- rhizosphere soil of okra as well as to evaluate the solubilization efficacy and the effectiveness of that strains in the presence of Tea dust, Eggshells, Bone waste of animal (ETB) and Rock Phosphate (RP) as an alternative fertilizer instead of chemical fertilizer with the reference of Triple Super Phosphate (TSP) as a control
The present study showed that PSB consortia were capable of solubilizing phosphate sources which contribute to promote the growth of Okra (Abelmoschus esculentus), there was a significant increase in the growth parameters compared to control treatment without consortia

Summary

Introduction

Okra (Abelmoschus esculentus) is an essential vegetable crop which supplies greater nutrition consists of protein, fats, carbohydrates, vitamins and minerals [1]. Phosphorus resources are limited and recycling phosphorus within the food industry is crucial for sustainable production This sort of mineral fertilizer is known as meat and bone meal (MBM). Root colonization by potential PSB tend to improve existing root growth length or by auxin hormone production from indole-3acetic-acid by the plant [15] This present study was aimed to isolate potential phosphate solubilizing bacteria (PSB) from rhizosphere and non- rhizosphere soil of okra as well as to evaluate the solubilization efficacy and the effectiveness of that strains in the presence of Tea dust, Eggshells, Bone waste of animal (ETB) and Rock Phosphate (RP) as an alternative fertilizer instead of chemical fertilizer with the reference of Triple Super Phosphate (TSP) as a control

Methods

Results

Conclusion