Evaluation of porcine semen quality by portable and desktop CASA systems - Short communication.

Eszter Balogh,Anna Boglárka Dálnoki,Orsolya Varga-Balogh,Viktória Johanna Debnár,Attila Zsolnai,József Rátky,István Anton,László Rózsa

doi:10.1556/004.2020.00023

Abstract

When using artificial insemination in porcine reproduction, one of the most important requirements is the suitable quality of semen regarding its total motility (TM) and progressive motility (PM). Computer-assisted sperm analysis (CASA) is an appropriate method to analyse the quality of semen. Recently a portable instrument has been developed to help specialists in their everyday field work. In our study, semen quality was measured simultaneously by the portable device (Ongo) and a laboratory CASA system (Microptic) to compare TM and PM values obtained by these appliances at a concentration of 50 × 106 spermatozoa/mL. Agreement between measurements was evaluated with a Bland-Altman plot. Strong correlation was found between the investigated instruments for all the three parameters, i.e. sperm concentration, TM and PM. However, a few measurements fell outside the defined range of acceptance.

Highlights

For a successful artificial insemination (AI) all quantity and quality parameters of semen and sperm cells have to be examined
When using artificial insemination in porcine reproduction, one of the most important requirements is the suitable quality of semen regarding its total motility (TM) and progressive motility (PM)
Semen quality was measured simultaneously by the portable device (Ongo) and a laboratory Computer-assisted sperm analysis (CASA) system (Microptic) to compare TM and PM values obtained by these appliances at a concentration of 50 3 106 spermatozoa/mL

Summary

Introduction

For a successful artificial insemination (AI) all quantity and quality parameters of semen and sperm cells have to be examined. Computer-assisted sperm analysis (CASA) is an appropriate method to analyse the quality of semen. Semen quality was measured simultaneously by the portable device (Ongo) and a laboratory CASA system (Microptic) to compare TM and PM values obtained by these appliances at a concentration of 50 3 106 spermatozoa/mL.

Results

Conclusion