Evaluation of Pomegranate Pomace Supplemented with Different Levels of Polyethylene Glycol using In Vitro Gas Production Technique

Abstract

The object of this study was to examine the chemical composition, including the tannin content of pomegranate pomace supplemented withdifferent levels of tannin-binding Agent (Polyethylene Glycol) and gas production amount using in vitro gas production technique. The treatment contained 0, 75, 150, 300 and 450mg PEG per each serum bottle, respectively. Approximately 300 mg of dried and ground (2mm) Pomegranate pomace were weighed and placed into serum bottles. CP, ADF, NDF, EE, ASH, TP and TT contents in pomegranate pomace were 8.7%, 27.6%, 32.3%, 1.7%, 9.2%, 5.4% and 4.6%, respectively. At the 2 h incubation times, the gas production amount of PP, PP + 75 mg PEG, PP + 150 mg PEG, PP + 300 mg PEG and PP + 450mg PEG were 29.83, 32.89, 23.26, 25.83 and 19.37 ml/g DM. At the first incubation times (2 and 4h), the PP + 75mg PEG treatments had the highest in vitro gas production amount within treatments (P<0.05). At all incubation times PP+75mg PEG treatment had the highest in vitro gas production amount within treatments (P<0.05). The addition of PEG at levels 75 and 450mg increased the in vitro gas production amount.