Abstract
The development of scaffolds mimicking the extracellular matrix containing bioactive substances has great potential in tissue engineering and wound healing applications. This study investigates melatonin—a methoxyindole present in almost all biological systems. Melatonin is a bioregulator in terms of its potential clinical importance for future therapies of cutaneous diseases. Mammalian skin is not only a prominent melatonin target, but also produces and rapidly metabolizes the multifunctional methoxyindole to biologically active metabolites. In our methodology, chitosan/collagen (CTS/Coll)-contained biomaterials are blended with melatonin at different doses to fabricate biomimetic hybrid scaffolds. We use rat tail tendon- and Salmo salar fish skin-derived collagens to assess biophysical and cellular properties by (i) Fourier transform infrared spectroscopy—attenuated total reflectance (FTIR–ATR), (ii) thermogravimetric analysis (TG), (iii) scanning electron microscope (SEM), and (iv) proliferation ratio of cutaneous cells in vitro. Our results indicate that melatonin itself does not negatively affect biophysical properties of melatonin-immobilized hybrid scaffolds, but it induces a pronounced elevation of cell viability within human epidermal keratinocytes (NHEK), dermal fibroblasts (NHDF), and reference melanoma cells. These results demonstrate that this indoleamine accelerates re-epithelialization. This delivery is a promising technique for additional explorations in future dermatotherapy and protective skin medicine.
Highlights
Licensee MDPI, Basel, Switzerland.The skin is a barrier organ that separates the body from the environment, protecting against microbial, physical, and chemical insults
We focused on the assessment of the presence of inexpensive, sensitive, and highly reproducible physicochemical tool for the characterifunctional groups within melatonin-enriched chitosan (CTS)/collagen (Coll)
Following an earlier study of Kaczmarek et al [62], we investigated different scaffolds based on collagen derived from rat tail tendon or from Salmo salar fish skin mixed with chitosan
Summary
Licensee MDPI, Basel, Switzerland.The skin is a barrier organ that separates the body from the environment, protecting against microbial, physical, and chemical insults. (N-acetyl-5-methoxytryptamine) is a ubiquitous physiological mediator that exists throughout the evolutionary scale of animals, plants, and unicellular organisms [1,2,3,4,5]. In mammals, it is most often characterized as a natural neurohormone synthesized in the pineal gland, from which it is released to modulate circadian rhythms [4]. Many other tissues, and cells, including bone marrow [6], lymphocytes [7], retina [8], astrocytes [9], thymus [10], skin [11,12,13], and female reproductive organs (granulosa cells, cumulus cells, and oocytes) [14], synthesize melatonin. MT1 and MT2 are high-affinity receptors for melatonin with
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