Evaluation of Plasmatic Procalcitonin in Healthy, and in Systemic Inflammatory Response Syndrome (SIRS) Negative or Positive Colic Horses.

Irene Nocera,Francesca Bonelli,Valentina Meucci,Micaela Sgorbini,Valentina Vitale,Giuseppe Conte,Eduard Jose-Cunilleras,Luis Alfonso Gracia-Calvo

doi:10.3390/ani11072015

Abstract

Simple SummaryProcalcitonin (PCT) increased in the case of systemic inflammatory response syndrome (SIRS), especially due to bacterial infection. The correlation between SIRS score and plasma PCT levels in horses have not been evaluated, and no studies investigated plasma PCT concentration over time. In the present study, PCT and SIRS score were evaluated in colic horses at admission to the hospital and at 24, 48, 72 and 96 h. Statistically differences were detected between healthy vs. all colic horses and between healthy vs. SIRS positive or SIRS negative horses. No correlation was observed between SIRS score and PCT. This suggests a role of plasmatic PCT as good biomarker for colic.Colic horses show systemic inflammatory response syndrome (SIRS) clinical signs. Procalcitonin (PCT) showed increased circulating levels in sick horses. This study compares plasma PCT concentrations in healthy vs. SIRS negative/positive colic horses over time, and evaluates PCT and SIRS score potential correlation, to verify the usefulness of PCT for the evaluation of SIRS severity. Ninety-one horses were included; 43/91 were healthy, on basis of physical examination, blood work and SIRS score (score = 0), while 48/91 were sick colic horses, classified as SIRS-negative (score < 2) and positive (score ≥ 2). Moreover, a 0–6 point-scale SIRS score was calculated (assessing mucous membrane color and blood lactate concentration). PCT was evaluated at admission, and at 24, 48, 72 and 96 h, using a commercial kit for equine species. We verified by the ANOVA test PCT differences between healthy vs. colic horses, healthy vs. SIRS-negative or SIRS-positive colic horses, at all sampling times, and the correlation between the SIRS score at admission with the SIRS score. Statistically significant differences were detected between healthy vs. all colic horses and between healthy vs. SIRS-positive or negative horses at all sampling times. No correlation was observed between the SIRS score at admission and PCT values. PCT was statistically higher in colic horses compared to the healthy ones, suggesting a role as a biomarker for colic.

Highlights

Colic is a term used to describe abdominal pain and represents the most common reason for emergency veterinary treatment
Many horses with colic show clinical signs similar to those described for systemic inflammatory response syndrome (SIRS) [2]
Previous studies indicate that the clinical pattern associated with inflammatory intestinal diseases or strangulating obstructions are more accurately described by the term SIRS instead of endotoxemia [2,3]

Summary

Introduction

Colic is a term used to describe abdominal pain and represents the most common reason for emergency veterinary treatment. Acute gastrointestinal disease is the most common cause of colic in horses, but a wide range of different diseases affecting the abdominal organs, such as urinary or genital tract disorders, can be reasons for horses showing signs of colic [1]. Many horses with colic show clinical signs similar to those described for systemic inflammatory response syndrome (SIRS) [2]. Previous studies indicate that the clinical pattern associated with inflammatory intestinal diseases or strangulating obstructions are more accurately described by the term SIRS instead of endotoxemia [2,3].

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