Evaluation of Piper betle mediated silver nanoparticle in post-harvest physiology in relation to vase life of cut spike of Gladiolus

Tilak Raj Maity,Aveek Samanta,Siraj Datta,Babita Saha

doi:10.1186/s42269-019-0051-8

Tilak Raj Maity, Aveek Samanta + Show 2 more

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https://doi.org/10.1186/s42269-019-0051-8

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Abstract

BackgroundThe purpose of this study is to evaluate the effect of green synthesized silver nanoparticles on post-harvest physiology of cut flower in relation to improvement of its vase life as well as keeping quality of the flowers taking gladiolus cut spikes as a model.ResultsThe synthesized Piper betle mediated silver nanoparticles (PbSNPs) were characterized by using UV-visible spectroscopy and transmission electron microscopy studies. It was revealed that PbSNPs ranges from 30 to 50 nm in diameter and spherical in shape. The experiments were conducted to study the effect of PbSNPs on post-harvest physiology, vascular blockage, prolonging vase life, and keeping the quality of cut spike of gladiolus. The treatment consisted of six vase solutions, viz. distilled water (control), 4% sucrose (T1), 4% sucrose + 100 ppm 5-SSA (T2), 4% sucrose + 2 ppm PbSNPs (T3), 4% sucrose + 4 ppm PbSNPs (T4), and 4% sucrose + 6 ppm PbSNPs (T5). Experimental outcomes depicted that T4 vase solution gives the best results in all the aspects statistically compared to its positive control (T2). The results also show PbSNPs in vase solution might have played a significant role for scavenging the reactive oxygen species by inducing antioxidant enzyme system at the time of senescence, leading to a decrease in lipid peroxidation and increase in the membrane stability.ConclusionsThis study revealed that application of T4 vase solution helps to maintain spikes fresh and dry weight, reduce the vascular blockage, improve the antioxidative defense, and stabilize the membrane integrity that leads to delay senescence.

Highlights

The purpose of this study is to evaluate the effect of green synthesized silver nanoparticles on postharvest physiology of cut flower in relation to improvement of its vase life as well as keeping quality of the flowers taking gladiolus cut spikes as a model
Phyto-synthesis of Piper betle silver nanoparticles (PbSNPs) and its characterization The aqueous solution of silver nitrate from colorless to yellowish brown indicates the synthesis of silver nanoparticles using ethanolic leaf extracts of Piper betel
The formation of PbSNPs is indicated by reduction of silver ion which leads to the color change (Fig. 1c (ii))

Summary

Introduction

The purpose of this study is to evaluate the effect of green synthesized silver nanoparticles on postharvest physiology of cut flower in relation to improvement of its vase life as well as keeping quality of the flowers taking gladiolus cut spikes as a model. Nanotechnology deals with the production and stabilization of various types of nanoparticles. It is a rapidly expanding research field which has tremendous implications on the society, industry, and medicine (Naik et al 2002; Kemp et al 2009). The silver nanoparticle synthesis using plant extracts is preferred as this method is non-toxic, cost-effective, eco-friendly, and easy to scale up in contrast to the chemical procedures which use hazardous material causing adverse effects to this approach (Dubey et al 2010; Kaushik et al 2010; Solgi and Taghizadeh 2012; Solgi 2014). Several groups of researchers have synthesized silver nanoparticles using a variety of available plants like banana (Bankar et al 2010), aloe (Chandran et al 2006), pomegranate and damask rose (Solgi and Taghizadeh 2012), geranium leaves (Shankar et al 2003), and basil (Ahmad et al 2010)

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