Abstract
Local selective phytovirological studies of plantations of pome fruits (apples, pears, quinces) for the presence of chlorotic maculation virus of the leaves of apple tree, apple tree mosaic virus, apple tree bark striation and pit virus in private and state agricultural enterprises of the Chernivtsi Region with a total area of 135.05 ha were carried out. Based on the results of visual local selective phytovirological studies, no signs of exposure to the studied viruses were found. The results of selective studies were confirmed via diagnostics of studied samples by ELISA.
Highlights
According to European conditions [10], study of agrocenosis was conducted from May to June, using young completely open leaves for the presence of apple tree mosaic virus (ATMV) and chlorotic maculation virus of apple leaves and apple on the old leaves for detection of apple tree bark striation and pit virus
Diagnostics of planting of pome fruits was carried out by the method of enzyme immunoassay (ELISA) using a test system manufactured by Loewe (Germany)
The principle double antibody sandwich (DAS) ELISA is to ensure that the microwellspecific antibodies immobilized on the surface provide capture of the virus, which is identified if present in the sample
Summary
At routine inspections in gardens with an area of up to 100 ha, at least 50 trees of two or three district varieties of main breeds were inspected. Each tree was examined from 4 sides, especially from the north, where the symptoms are more clearly manifested and persisted longer in mother fruit planting. The total inspection area was 135.05 ha, of which apple plantations – 128.05 ha and pears – 7 ha. Samples of the following apple tree varieties were selected: Lisa, Florina, Chempion, Aidared, Jonathan Gold, Golden Delicious, Gloucester, Fuji, Renet, Symyrenka, and pear tree: Noiabrska, Snizhynka, Krupnoplidna
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