Abstract
In India, a wide variety of medicinal plants are reported and utilized by people for the treatment of various diseases for a long time. The present study deals with quantitative analysis of phytochemicals like total phenols, tannins, and flavonoids as well as in-vitro antioxidant and anti-inflammatory activity of ethanolic extract of Ocimum basilicum L. The values of total phenols, tannins, and flavonoids were found to be 5.02±0.06 µg Gallic acid equivalent/mg, 7.80±0.05 µg Gallic acid equivalent/mg, and 6.00±0.06 µg quercetin equivalent/mg alcoholic extract respectively. The antioxidant activity was measured by DPPH (2, 2-diphenyl-1-picryl-hydrazyl-hydrate) assay. The highest antioxidant activity of plant extract was observed at 60 µg/ml and maximum inhibition was recorded at 55.12%. The IC50 value of plant extract was found to be 24.81 µg/ml. In-vitro anti-inflammatory activity was measured by the human red blood cell (HRBC) membrane stabilization method. The hypo tonicity-induced HRBC were exposed to different concentrations of ethanolic extract of Ocimum basilicum L. and HRBC membrane lysis and membrane stabilization percentages were calculated against diclofenac sodium. The ethanolic extract exhibited significant HRBC membrane stabilization compared to diclofenac sodium; 98±0.57% membrane stabilization was observed at a dose of 1000 µg/ml.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
