EVALUATION OF PHYTOCHEMICALS AND NEUROLEPTIC POTENTIAL OF LEAVES EXTRACT OF ADENIUM OBESUM

Abstract

The term psychosis refers to illnesses that impair the mind and cause a loss of touch with reality. The present research focuses on screening of phytochemicals and neuroleptic potential of leaves extract of Adenium Obesum in animal models. Leaves of Adenium obesum were obtained from the Saharanpur, UP region and these were identified and authenticated by a botanist at BSI, (Prayagraj) Allahabad. The leaves were washed making dust-free and dried at room temperature or shade. The dried leaves were rendered into coarse powders and then finally into fine ones. The powder was weighed and soaked into hydroalcoholic solution consisting ethanol+ distilled water (1:1) for fifteen days with gradual stirrings. A rotating evaporator is used to dry the brownish until a semisolid extract was obtained. Animal House, Department of Pharmacy, Glocal University Saharanpur (UP) was provided Wistar rats of either sex weighing 130–160g. Animals were divided in 4 groups (n=6) i.e., group1 administered only normal saline daily for 21 days, group 2 administered pregabalin (10mg/kg) orally, for 21 days, group 3 administered hydroalcoholic leaves extract of Adenium obesum (HLAO) at dose of 200mg/kg orally, for 21 days and group 4 administered hydroalcoholic leaves extract of Adenium obesum (HLAO) at dose of 400mg/kg orally up to 21 days. Screening of phytochemicals and then neuroleptic activity of Adenium Obesum by screening models i.e., Apomorphine-induced stereotypy, Haloperidol-induced catalepsy and Climbing behavior test was evaluated. In results, Adenium obesum has prominent neuroprotective effect at lower and higher doses (200mg/kg & 400mg/kg). But response was based on dose levels. It exhibited a significant neuroprotective behavior it might be due to better release of constituents from the leaves of Adenium obesum plant. In conclusion, Adenium obesum has potential neuroprotective behaviour at the all the doses used but highest was observed at higher dose of ethanolic extract that might be due to better solubility and constituents release from the A. obesum leaves in extraction. Future research suggests, to identify and isolate the responsible element for the pharmacological potential and make the suitable dosage form.