Abstract
The aim of this study was to determine the antioxidant capacity of some phytochemicals by using an H2O2 scavenging assay. Betaine, Allantoin and Nicotinamide were put to the test. Even though hydrogen peroxide (H2O2) is not intrinsically reactive, it can be transformed into the extremely reactive and harmful hydroxyl radical (HO), which is then able to interact with nucleotides in deoxyribose nucleic acid and in that way trigger breakage of the strand resulting in carcinogenesis, mutagenesis, etc. Antioxidants aid in the protection of cells from the harmful effects of reactive oxygen species that are known to induce oxidative stress. Excessive production of these reactive oxygen species in the human body is associated with many chronic degenerative diseases such as diabetes, neurodegenerative disease, cancer, etc. The effective way to minimize levels of oxidative stress is the ability to scavenge these reactive oxygen species (ROS). Also, phytochemicals are able to act as antioxidants, and in that way play a vital role in the prevention of disease caused by oxidative stress. The ability of a compound to scavenge H2O2 is a good predictor of its potential antioxidant function. The hydrogen peroxide (H2O2) scavenging assay was determined using the Ruch et al., 1989 method, and a UV-VIS spectrophotometer. In conclusion, our samples had marvellous H2O2 scavenging activity and possessed good antioxidant capability, and were compared with the ascorbic acid (vitamin c) as standard natural antioxidant/ as reference antioxidant. The samples were also able to scavenge H2O2 in a concentration-dependent way, according to our results
Highlights
1.1 Reactive oxygen speciesROS, or reactive oxygen species, refers to a wide range of compounds and free radicals emanated from molecular oxygen [1]
This study aimed to evaluate the ability of phytochemicals to scavenge H2O2 concentration
2.2 Hydrogen peroxide scavenging activity The hydrogen peroxide scavenging assay was determined according to Ruch et al method [29]. 10 mg of allantoin, betaine and nicotinamide were weighed on a balance and diluted in 1000 of distilled water
Summary
ROS, or reactive oxygen species, refers to a wide range of compounds and free radicals emanated from molecular oxygen [1]. Free radicals including superoxide anion radicals (O2-), hydroxyl radicals (OH·), and non-free radical species including hydrogen peroxide (H2O2) and singlet oxygen (1O2) are examples of ROS [2]. Free radicals can be formed from endogenous and exogenous sources. Endogenous can be inflammation, cancer, mental stress, infection, ageing. While exogenously free radicals can penetrate the body and degrade due to exposure to heavy metals, such as mercury, lead, iron, some environmental pollutants, or drugs.
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