EVALUATION OF PHYTOCHEMICAL AND PHARMACOLOGICAL ACTIVITY OF METHANOLIC EXTRACT OF SOLANUM PUBESCENS

Abstract

Traditional medicine has a long history of serving people all over the world. In recent years, the use of traditional medicine information in cancer research received considerable interest. Solanum pubescens has been used in traditional and folklore medicine for the treatment of cancer. The aim of the present study was to evaluate the effect of methanol extract of the leaves against intraperitoneally injected Dalton’s Ascitic Lymphoma (DAL) cell line in swiss albino mice. DAL cells were injected intraperitoneally (1 ×10 cells / ml / mouse) to the mice. The MESP at a dose of 100 mg / kg and 200 mg / kg body weight were administered orally for 14 consecutive days to the tumor bearing group of animals. Derived parameters, haematological parameters, serum enzyme and lipid parameters were measured and compared to the cancer control group. 5Flurouracil (20 mg / kg) was used as a standard. Both the dose of MESP decreased average increase in body weight, reduced the packed cell volume, viable tumor cell count and increased the life span of DAL treated mice and brought back the haematological parameters, serum enzyme and lipid profile near to normal values. All the values were found to be statistically significant with cancer control group at P < 0.01. These observations are suggestive of the protective effect of extracts in Dalton’s Ascitic Lymphoma (DAL). All these findings enable to conclude that MESP at 200 mg / kg dose possess a protective effect against DAL.