Evaluation of Phytochemical Analysis and Antimicrobial Activity of Citrus Latifolia Peel Extract

Abstract

Citrus latifolia accounts for its phytochemical activity, these bioactive compounds result in the anti-oxidative, anti-inflammatory, anti-microbial effects that can serve mankind. The aim of the present work investigates the presence of the bioactive components present in the Methanol extracts of the Citrus latifolia peel and examines its potent antimicrobial and antioxidant activity. The plant material was dried and extracted in the soxhlet extractor and was concentrated using rotary evaporator. The extract was then tested for the anti-oxidant activity using DPPH assay and antimicrobial activity using well diffusion method. The extract was then lyophilized and analyzed for the presence of potent bioactive compounds using GCMS technique. The DPPH antioxidant profiling of the methanol extract of Citrus latifolia peel showed maximum radical scavenging activity compared with the IC50 of Ascorbic acid. The extract showed inhibitory antimicrobial activity against Escherichia coli, Pseudomonas aeruginosa, streptococcus pyogenes, and Listeria monocytogenes. The GC-MS analysis reveals the presence of eighteen compounds such as alcohol, acid-esters, aromatic acids, furanone, polyester, coumarins, hypoxanthine, that is responsible for the antioxidation and anti-microbial effect of the Citrus latifolia. The investigation from this study has concluded that the methanol extract of Citrus latifolia will be the alternative medication to such threat full pathogens.