Abstract
Bicarbonate species in the aqueous phase is the primary source for CO2 for the growth of microalgae. The potential of carbon dioxide (CO2) fixation by Chlorella pyrenoidosa in enriched bicarbonate medium was evaluated. In the present study, effects of parameters such as pH, sodium bicarbonate concentration and inoculum size were assessed for the removal of CO2 by C. pyrenoidosa under mixotrophic condition. Central composite design tool from response surface methodology was used to validate statistical methods in order to study the influence of these parameters. The obtained results reveal that the maximum removal of CO2 was attained at pH 8 with sodium bicarbonate concentration of 3.33 g/l, and inoculum size of 30 %. The experimental results were statistically significant with R 2 value of 0.9527 and 0.960 for CO2 removal and accumulation of chlorophyll content, respectively. Among the various interactions, interactive effects between the parameters pH and inoculum size was statistically significant (P < 0.05) for CO2 removal and chlorophyll accumulation. Based on the studies, the application of C. pyrenoidosa as a potential source for carbon dioxide removal at alkaline pH from bicarbonate source is highlighted.
Highlights
Rapid exploitation of fossil fuels such as coal, petroleum, etc., has invariably increased the concentration of CO2 in the atmosphere to *400 ppm (Rahaman et al 2011)
The potential of carbon dioxide (CO2) fixation by Chlorella pyrenoidosa in enriched bicarbonate medium was evaluated. Effects of parameters such as pH, sodium bicarbonate concentration and inoculum size were assessed for the removal of CO2 by C. pyrenoidosa under mixotrophic condition
It can be noted that, there was a twofold increase in the chlorophyll accumulation in the optimized condition compared to the experimental condition which may be due to the availability of carbon source that helps in synthesizing chlorophyll
Summary
Rapid exploitation of fossil fuels such as coal, petroleum, etc., has invariably increased the concentration of CO2 in the atmosphere to *400 ppm (Rahaman et al 2011). Biofixation of CO2 by microalgae attracted researchers due to its affirmative behavior such as carbon neutral and biomass productivity for its various applications. The factors such as economic aspects, life cycle analysis, capital investment and other environment aspects have made these technological impacts slower at commercial level. PH is the major determinant of the relative concentrations of the carbonaceous system species in water and could affect the availability of carbon for algal photosynthesis in intensive cultures and at certain situations where the supply of adequate CO2(g) is limited, inorganic carbon sources such as bicarbonate salts (NaHCO3) is best alternative to cultivate microalgae (Hsueh et al 2009; Lam and Lee 2013; Benemann 1993).
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
