Abstract
We report results of our studies on cellular uptake and phototoxicity of free chlorin p6 (Cp6) and Cp6 bound to amine-modified silica nanoparticles (Cp6-SiNP) in colon (Colo-205) and oral cancer (Nt8e) cell lines. Fluorescence spectroscopy-based measurement of cellular uptake showed no significant difference in the amount of free Cp6 and Cp6-SiNP localized in cells. Measurements on phototoxicity by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that the Cp6-SiNP was more effective as compared with free Cp6. Fluorescence-correlation spectroscopy performed on the free drug and drug-SiNP complex in serum media confirmed that the drug remains preferentially bound to SiNP. The rate of photobleaching was much lower in case of Cp6-SiNP complex as compared with the free drug in serum media. These results suggest that the observed increase in photodynamic activity of Cp6-SiNP complex may arise because of an increase in the photostability of Cp6 when bound to SiNP.
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