Abstract

The chemical composition and bioactivity of green coffee is influenced by both intrinsic and extrinsic factors, and it is a rich source of bioactive compounds, including phenolic compounds, alkaloids, and many compounds with antioxidant and anti-inflammatory properties. These characteristics have sparked numerous studies on its chemical composition and, consequently, have been attributed numerous advantages and disadvantages for human health. This study aimed to quantify the total phenolic constituents and analyze the in vitro antioxidant activity of green coffee from the Conquista Plateau region, Bahia, Brazil, and separate them according to their antioxidant potential. The total phenolic content (TPC) was determined and the evaluation of the in vitro antioxidant potential was investigated by the DPPH radical scavenging methods and the β-carotene:linoleic acid (BCLA) system co-oxidation method. Principal component analysis (PCA) was applied to reveal which samples showed higher and lower antioxidant potential. The results showed that the samples presented moderate content of total phenolic constituents 27.02 mg. g-1 of sample, low primary antioxidant activity with the average value of 65.17 EC50 mg. g-1 sample and moderate secondary antioxidant activity with 44% protection from oxidation of the β-carotene molecules present, moderately inhibiting the conditions favorable to oxidation that were inserted during the analysis. The principal components analysis proved efficient in the separation and classification of the green coffee samples, allowing the observation of the dispersion and heterogeneity among the samples, demonstrating different composition and bioactivity profiles.

Highlights

  • The coffee bean is a unique product of high economic and historical importance since the days of the café au lait policy

  • Called raw coffee, characterized by the unroasted seeds of the fruit, has beneficial properties that contribute to an increased interest in incorporating it into various food products (Świeca et al, 2017)

  • Proteins, glycides, minerals, besides several components such as trigonelline acids and caffeine that contribute to the prevention of the development of diseases

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Summary

Introduction

The coffee bean is a unique product of high economic and historical importance since the days of the café au lait policy. The chemical composition of green coffee beans is influenced by intrinsic and extrinsic factors in a comprehensive way, such as its species, variety, soil condition, altitude, climate, cultivation and processing methods, varying according to the region (Lucci, Pacetti & Frega, 2015). It consists of lipids, proteins, glycides, minerals, besides several components such as trigonelline acids and caffeine that contribute to the prevention of the development of diseases

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