Evaluation of PC12 Cells' Proliferation, Adhesion and Migration with the Use of an Extracellular Matrix (CorMatrix) for Application in Neural Tissue Engineering.

Katarzyna Gębczak,Wojciech Fortuna,Benita Wiatrak

doi:10.3390/ma14143858

Katarzyna Gębczak, Wojciech Fortuna + Show 1 more

Open Access

https://doi.org/10.3390/ma14143858

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Journal: Materials	Publication Date: Jul 10, 2021
Citations: 2	License type: CC BY 4.0

Affiliation: Wroclaw Medical University

Abstract

The use of extracellular matrix (ECM) biomaterials for soft tissue repair has proved extremely successful in animal models and in some clinical settings. The aim of the study was to investigate the effect of the commercially obtained CorMatrix bioscaffold on the viability, proliferation and migration of rat pheochromocytoma cell line PC12. PC12 cells were plated directly onto a CorMatrix flake or the well surface of a 12-well plate and cultured in RPMI-1640 medium and a medium supplemented with the nerve growth factor (NGF). The surface of the culture plates was modified with collagen type I (Col I). The number of PC12 cells was counted at four time points and then analysed for apoptosis using a staining kit containing annexin V conjugate with fluorescein and propidium iodide (PI). The effect of CorMatrix bioscaffold on the proliferation and migration of PC12 cells was tested by staining the cells with Hoechst 33258 solution for analysis using fluorescence microscopy. The research showed that the percentage of apoptotic and necrotic cells was low (less than 7%). CorMatrix stimulates the proliferation and possibly migration of PC12 cells that populate all levels of the three-dimensional architecture of the biomaterial. Further research on the mechanical and biochemical capabilities of CorMatrix offers prospects for the use of this material in neuro-regenerative applications.

Highlights

The fundamental part of tissue stroma is the extracellular matrix (ECM)
After treatment with nerve growth factor (NGF), these cells become similar to sympathetic neurons, and it has been recognized that they can be a model in neurobiological research
This study suggests possible greater cell migration to CorMatrix than to the surface modified with type I collagen

Summary

Introduction

The fundamental part of tissue stroma is the extracellular matrix (ECM). ECM is the release product of cells settling on a given tissue; that is why both the composition and distribution of ECM particles depend on the tissue that ECM is derived from [1,2]. ECM maintains the particular tissue phenotype; influences the functioning of numerous regulatory growth factors and the availability of chemokines; and has a significant impact on cell survivability, proliferation and cell differentiation [3]. Inside ECM, there are ligands stimulating integrins and other cell surface receptors, influencing the intercellular signalling pathways [1]. The history of using native ECM proteins in the construction of biomaterials starts with coating biomaterial surfaces with purified proteins, e.g., medicinal product Biobrane® , which was a synthetic network bonded with pig collagen [4].

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