Abstract
Uropathogenic Escherichia coli (UPEC), an important causative agent of urinary tract infections (UTIs), carries virulence factors which are clustered on pathogenicity islands (PAIs). The goal of this study was to characterize the PAIs among the UPEC isolated from patients with urinary catheters. A descriptive cross-sectional study was designed and from December 2014 to April 2015, 78 non-duplicate E. coli were collected from hospitalized patients with UTIs, including patients with and without indwelling urinary catheters. Two multiplex polymerase chain reaction (PCR) assays were performed to evaluate the presence of the eight most studied PAIs (I 536, II 536, III 536, IV536, I CFT073, II CFT073, PAI IJ96, and PAI IIJ96). Of 78 patients with a UTI 31 (39.7%) used indwelling catheters. Of these 31 patients, 27 (87.1%) carried PAIs markers, including 25 (80.6%) PAI IV536, 12 (38.7%) PAI ICFT073, 6 (19.4%) PAI IICFT073, 6 (19.4%) PAI III536 and 3 (9.7%) PAI II536. PAI I536, PAI IJ96, and PAI IIJ96 were not detected in the UPEC strains. The findings of this study revealed that the frequency of PAI markers in UPEC isolates from patients with indwelling urinary catheters was high. The rate of multiple PAIs carriage was notable among those patients, suggesting that UPEC strains that colonize the indwelling urinary catheters have the potential to cause complicated urinary infections. PAI ICFT073, which was found in association with pyelonephritis, prostatitis, and sepsis, could be considered as a target for medical interventions.
Highlights
Uropathogenic Escherichia coli (UPEC), an important causative agent of urinary tract infections (UTIs), carries virulence factors which are clustered on pathogenicity islands (PAIs) .The goal of this study was to characterize the PAIs among the UPEC isolated from patients with urinary catheters
PAIs are mobile genetic elements that provide a strong route for horizontal transfer of virulence genes, which is why these DNA regions are involved in bacterial evolution, especially among pathogenic bacteria [9]
Of the 78 UPEC isolates, 31 (39.7%) were from patients with indwelling urinary catheters and 47 (60.3%) were from patients without urinary catheters
Summary
Uropathogenic Escherichia coli (UPEC), an important causative agent of urinary tract infections (UTIs), carries virulence factors which are clustered on pathogenicity islands (PAIs) .The goal of this study was to characterize the PAIs among the UPEC isolated from patients with urinary catheters. Conclusions: The findings of this study revealed that the frequency of PAI markers in UPEC isolates from patients with indwelling urinary catheters was high. The rate of multiple PAIs carriage was notable among those patients, suggesting that UPEC strains that colonize the indwelling urinary catheters have the potential to cause complicated urinary infections. Several different virulence factors necessary for the establishment of those in the urinary tract and causing infection have been recognized in UPEC strains [6,7]. Very little is known about PAI markers of UPEC strains isolated from
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