Abstract
BACKGROUND TcP21 is a ubiquitous secreted protein of Trypanosoma cruzi and its recombinant form (rP21) promotes parasite cell invasion and acts as a phagocytosis inducer by activating actin polymerisation in the host cell.OBJECTIVE Our goal was to evaluate if the additional supplementation of rP21 during a prime/boost/challenge scheme with T. cruzi TCC attenuated parasites could modify the well-known protective behavior conferred by these parasites.METHODS The humoral immune response was evaluated through the assessment of total anti-T. cruzi antibodies as well as IgG subtypes. IFN-γ, TNF-α and IL-10 were measured in supernatants of splenic cells stimulated with total parasite homogenate or rP21.FINDINGS Our results demonstrated that, when comparing TCC+rP21 vs. TCC vaccinated animals, the levels of IFN-γ were significantly higher in the former group, while the levels of IL-10 and TNF-α were significantly lower. Further, the measurement of parasite load after lethal challenge showed an exacerbated infection and parasite load in heart and skeletal muscle after pre-treatment with rP21, suggesting the important role of this protein during parasite natural invasion process.MAIN CONCLUSION Our results demonstrated that rP21 may have adjuvant capacity able to modify the cytokine immune profile elicited by attenuated parasites.
Highlights
TcP21 is a ubiquitous secreted protein of Trypanosoma cruzi and its recombinant form promotes parasite cell invasion and acts as a phagocytosis inducer by activating actin polymerisation in the host cell
We know that recombinant P21 (rP21) promotes T. cruzi cell invasion,(13) invading parasites could freely multiply inside the host cell and invade other cells
RP21 could be acting as a phagocytosis inducer by activating actin polymerisation in the host cell [14] in this scenario, parasites could be confined within the invaded cell due to this phenomenon
Summary
TcP21 is a ubiquitous secreted protein of Trypanosoma cruzi and its recombinant form (rP21) promotes parasite cell invasion and acts as a phagocytosis inducer by activating actin polymerisation in the host cell. Anti-rP21 specific humoral response is abrogated under the presence of attenuated parasites - As a first instance we focused on determining the levels of total IgGs antibodies in sera from immunised animals.
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