Evaluation of partial thromboplastin time, thrombin time and prothrombin time over treated plasma using a fibrinolytic protease

Vanessa Maria Andrade Machado Miranda,Ana Cristina Lima Leite,Lorenzo Pastrana,Juanize Matias Da Silva Batista,Romero Marcos Pedrosa Brandão Costa,José Pedro Martins Barbosa Filho,Vagne De Melo Oliveira,Thiago Pajeú Nascimento,Ana Lúcia Figueiredo Porto

doi:10.33448/rsd-v11i2.25439

Abstract

Fibrinolytic proteases are enzymes that degrade the fibrin, a main component of the blood clot. Thus, the present study aimed to understand the evaluation of aPTT, TT and PT on plasma treated with a fibrinolytic protease produced by Mucor subtillissimus. To assess the anticoagulant effect, the protease was used in different concentrations of 0.5-2.5mg/mL. The tests showed that the enzyme promoted a significant prolonged time over the PT clotting time as concentration increased. In the aPTT assay, fibrinolytic protease practically did not prolong the clotting time, even with increased enzyme concentration. In TT it was verified that at all times of contact with thrombin, be it 5-30 minutes, there was no interference on the action of thrombin or fibrinogen on the formation of thrombi. Since only the prothrombin time among all those studied was altered, it is suggested that the fibrinolytic protease under study affects the extrinsic pathway of coagulation. Thus, it was possible to observe that the serine protease promoted a significant prolonged time on the clotting time of the PT as the enzyme concentration increased, but the serine protease did not cause any type of change in TT and aPTT.

Highlights

Cardiovascular diseases are the leading cause of death in the world
The tests showed that the fibrinolytic protease produced by Mucor subtilissimus UCP 1262 promoted a significant prolonged time over the Prothrombin Time (PT) clotting time as the enzyme concentration increased (Figure 1)
Similar results were observed by Sun et al (2015), where a significant prolonged increase in TP was observed in a manner dependent on the concentration of a fibrinolytic protease (AfeE) produced by Streptomyces sp

Summary

Introduction

Cardiovascular diseases are the leading cause of death in the world. It is estimated that 17.7 million people died from cardiovascular disease in 2015, representing 31% of all deaths globally. Of these deaths, it is estimated that 7.4 million occur due to cardiovascular diseases and 6.7 million due to strokes. More than three quarters of deaths from cardiovascular disease (CVDs) occur in low- and middle-income countries (Hu et al, 2019). Among the various types of CVDs, a thrombosis is among the most common causes of death today and may be responsible for the increase in the number of deaths (SBC, 2020). The formation of a clot in a blood vessel (intravascular thrombosis) is a major cause of CVDs (Kotb et al, 2012)

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