Evaluation Of Oxidative Stress On Dogs With Demodicosis

Abstract

Aim: In this study, it was aimed to investigate the effects of demodicosis on oxidative stress in dogs.
 Materials and Methods: The material of the study is based on a total of 32 dogs, 21 of which are diagnosed with demodicosis and 11 of which are healthy, with different ages, genders and breeds. Examination for diagnostic evaluation of demodex is performed by examining the samples under the microscope which are taken by trichogram and deep skin scraping methods. In order to evaluate the oxidative stress on dogs with demodicosis at pre and post treatment groups and the control group without demodex diagnosis, the values of SOD (Superoxide dismutase), GPX (Glutation Peroxidase), GSH (Glutation) as antioxidant and MDA (Malondialdehyde) as oxidant are investigated.
 Results ?: In clinical examinations, manifestations such as alopecia, erythema, generalized pruritus, hyperpigmentation, lichenification, pododermatitis, interdigital pruritus, lymphadenopathy are observed on dogs with demodicosis on pre and post treatment stages. In the analyzes performed in order to evaluate the oxidative stress, MDA 20.30 nmol/ml, GSH 4.9 nmol/ml, GPx 0.42 U/l, SOD 4.1 U/l are measured in dogs with clinical demodicosis. At the post treatment stage, the average values on the same dogs are measured as MDA 6.08 nmol/ml, GSH 8.11 nmol/ml, GPx 0.83 U/l, SOD 6.67 U/l, whilst in the control group they were measured as MDA 4.94 nmol/ml, GSH 9.73 nmol/ml, GPx 0.97 U/l, SOD 7.20 U/l. It is obtained that, GSH, GPx and SOD values in the control and post treatment groups are significantly higher (p˂0.001) and MDA value is lower (p˂0.001) than the clinical demodicosis group.
 Conslusion: In dogs with clinical demodicosis, compared to control and post-treatment, higher level of MDA which is oxidant and lower level of GSA, GPx and SOD which are antioxidants showed that demodex is caused oxidative stress.