Evaluation of opioid withdrawal after drug cessation in morphine and mitragynine dependent rats

Abstract

Event Abstract Back to Event Evaluation of opioid withdrawal after drug cessation in morphine and mitragynine dependent rats RAHIMAH HASSAN1, Sharif M. Mansor1 and Zurina Hassan1* 1 University of Science, Malaysia, Malaysia Background Mitragynine is an indole alkaloid found in Mitragyna speciosa or known as Kratom. It is native in Southeast Asia and originally used for its therapeutic effects and as a substitution in self-treatment of opiate addiction. However, mitragynine consumption develops drug dependence. Hence, the present study aims to evaluate drug dependence after drug cessation via spontaneous withdrawal symptoms in morphine and mitragynine dependent rats. Methods Morphine dependent rats were developed by increasing the doses (mg/kg and shown in bracket) twice daily for 6 consecutive days: 1st day (0, 10), 2nd day (10, 10), 3rd day (20, 20), 4th day (30, 30), 5th day (40, 40), 6th day (50, 50). Morphine treatment was disrupted on day 7 in order to induce spontaneous opiate abstinence. For mitragynine dependent rats, 30 mg/kg were given daily for 14 days and disrupted on day 15. The assessment of physical dependence of morphine, mitragynine and control groups were based on motor and vegetative signs. The signs of spontaneous withdrawal symptoms were recorded for 30 minutes in an open field test chamber, 24 hours after the last dose for 28 days. The 'counted signs' and 'checked signs' were multiplied with the respective 'weighing factors' for the evaluation of the severity of abstinence syndrome. Results The morphine dependent rats develop opioid withdrawal by significantly increase the mean aggregate value of spontaneous withdrawal symptoms. The morphine withdrawal symptoms last for 16 days after drug cessation. However, the spontaneous withdrawal symptoms of mitragynine dependent rats reduced by half on the 2nd day after drug cessation and last for 9 days. Conclusion Spontaneous withdrawal symptoms in mitragynine dependent rats was found to be resolved in shorten period compared to morphine dependence rats. Acknowledgements This project is funded by HICoE Ministry of Education Special Grant 304.CDADAH.4401009 Keywords: mitragynine, Morphine, Opioid withdrawal, Drug cessation, spontaneous withdrawal symptoms Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Poster Presentation Topic: Mental health Citation: HASSAN R, Mansor SM and Hassan Z (2019). Evaluation of opioid withdrawal after drug cessation in morphine and mitragynine dependent rats. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00071 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 30 Sep 2018; Published Online: 17 Jan 2019. * Correspondence: Dr. Zurina Hassan, University of Science, Malaysia, George Town, Malaysia, zurina_hassan@usm.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers RAHIMAH HASSAN Sharif M Mansor Zurina Hassan Google RAHIMAH HASSAN Sharif M Mansor Zurina Hassan Google Scholar RAHIMAH HASSAN Sharif M Mansor Zurina Hassan PubMed RAHIMAH HASSAN Sharif M Mansor Zurina Hassan Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.