Abstract
BackgroundThe dentin is a tissue, which is formed by odontoblasts at the pulp interface of the teeth that supports the enamel. Odontoblasts, the cranial neural crest cells are derived from ectodermal mesenchymal stem cells (MSCs) and are long and polarized cells. They are present at the outer surface of dentin and play a prominent role about dentin formation. Recently, attention has been focused on induction of odontoblast using various type of MSCs and effects of the 17ß-estradiol supplementation. In this study, we establish an efficient odonto/osteoblast differentiation protocol using 17ß-estradiol supplementation while comparing the odonto/osteoblast ability of various dental MSCs.MethodsSame donor derived four types of dental MSCs namely dental pulp stem cells (DPSCs), stem cells from apical papilla (SCAP), dental follicle stem cells (DFSCs), and periodontal ligament stem cells (PDLSCs) were evaluated for their stemness characteristics and potency towards odonto/osteoblast (Induced odonto/osteoblast) differentiation. Then 17ß-estradiol supplementation of 0 and 10 µM was applied to the odonto/osteoblast differentiation media for 14 days respectively. Furthermore, mRNA and protein levels of odonto/osteoblast markers were evaluated.ResultsAll of the experimental groups displayed stemness characteristics by showing adipocyte and chondrocyte differentiation abilities, expression for cell surface markers and cell proliferation capacity without any significant differences. Moreover, all dental derived MSCs were shown to have odonto/osteoblast differentiation ability when cultured under specific conditions and also showed positive expression for odontoblast markers at both mRNA and protein level. Among all, DPSCs revealed the higher differentiation potential than other dental MSCs. Furthermore, odonto/osteoblast differentiation potential was enhanced by supplementing the differentiation media with 17ß-estradiol (E2).ConclusionsThus, DPSCs possess higher odonto/osteogenic potential than the SCAPs, DFSCs, PDLSCs and their differentiation capacity can by further enhanced under E2 supplementation.
Highlights
The dentin is a tissue, which is formed by odontoblasts at the pulp interface of the teeth that supports the enamel
Isolation and characterization of mesenchymal stem cells (MSCs) derived from dental pulp, follicle, apical papilla, and periodontal ligament Dental-derived MSCs (DPSCs, Stem cells from apical papilla (SCAPs), dental follicle stem cells (DFSCs), and periodontal ligament stem cells (PDLSCs)) were isolated and cultured
The in vitro differentiation potentials of dental pulp stem cells (DPSCs), SCAP, DFSCs, and PDLSCs at passage three were evaluated by differentiating each cell source into adipocytes, and chondrocytes
Summary
The dentin is a tissue, which is formed by odontoblasts at the pulp interface of the teeth that supports the enamel. Odontoblasts, the cranial neural crest cells are derived from ectodermal mesenchymal stem cells (MSCs) and are long and polarized cells. They are present at the outer surface of dentin and play a prominent role about dentin formation. We establish an efficient odonto/osteoblast differentiation protocol using 17ß-estradiol supplementation while comparing the odonto/osteoblast ability of various dental MSCs. Dentin is a tissue that supports enamel and occupies most of the teeth. The caries, periodontal diseases and physical injuries, are the most frequently occurring tooth problems that cause dentin loss In this process, Son et al BMC Oral Health (2021) 21:15 odontoblasts are destroyed and induction of odontoblast-like cell from stem cells may prove helpful [1,2,3]. Many studies have focused on odontoblast differentiation using stem cells
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