Evaluation of Noxious Effect of Environmental Contaminants Using Zebrafish Model

Abstract

Effects of the inorganic chemicals Calcium Fluoride (CaF2) and Hexaflurosilicilic acid (H2SiF2) have been studied due to its excessive usage in drinking water plants, glass manufacturing etc. Toxicity studies on Zebrafish embryos have been carried out for CaF2 and H2SiF2 during the embryonic developmental stages to observe the changes taken place during the growth, development. These changes can be observed in cell differentiation, larval movements, delay in hatching, and by the changes in behavior. Due to the ease with the transparency of zebrafish embryos, it can be observed and manipulated. In the field of early developmental studies, these zebrafish embryos have been vital because they have faster development by which the whole organs get developed in 3 days. Thus it plays a significant role in the discovery and analysis of changes in the developmental aspects of their teratology study. Toxicity study in Adults Zebrafish can be studied through the histology analysis where the cell damage and cell death due to fluorides and acid ions which may also lead to morphological changes due to this environmental pollutant. This toxicity study can be studied based on behavioral effects, LC50 determination, and immunohistochemistry of the brain to observe the developmental neurotoxicity. This study describes the effect of the inorganic chemicals is leading to developmental toxicity, cell deformities, and cell death with the high mortality rate in the In vivo Zebrafish model.