Evaluation of Non-polar Composition in Plumbago Zeylanica Leaves by Gas Chromatography and Mass Spectrometry.

Jayesh Dhalani,Chirag Rathod,Gaurang Dubal,Pankaj Nariya

doi:10.3897/folmed.62.e47647

Abstract

Plumbago zeylanica plant belongs to Plumbaginaceae. The plant is reported for many pharmacological activities. The objective of the study was to identify fatty acids and non-polar chemical compounds in Plumbago zeylanica leaves. Petroleum ether extract was prepared using soxhlet apparatus. Saponifiable and unsaponifiable matter was separated with saponification process. To identify fatty acids in saponifiable matter further esterification was performed. Gas chromatography and mass spectrometry analysis was performed of both saponifiable and unsaponifiable fractions. All the fatty acid methyl esters and non-polar chemical compounds were identified using NIST library data. A total of 14 compounds were identified with comparison of NIST data. From that, 8 fatty acid methyl esters and 6 non-polar chemical compounds were identified. Here we have analyzed fatty acids and non-polar chemical compounds by the same GC-MS method. The present analysis showed that Plumbago Zeylanica leaves contain 8 fatty acids and 6 non-polar chemical compounds. Principal determination of the research was development of efficient method to identify non-polar compound from plant by single injection using chromatographic technique.

Highlights

Plumbago zeylanica is an important medicinal plant
The extract was dried over Na2SO4 and volume was made up to 10 ml with hexane and gas chromatography, and mass spectrometry analysis was performed
The result showed that by the esterification procedure eight fatty acids were identified by GC-MS

Summary

Introduction

Plumbago zeylanica is an important medicinal plant. Non-polar profile of plant leaves was still not reported. We are reporting for the first time non-polar compounds presence in plant leaves of Plumbago zeylanica. Aim: The objective of the study was to identify fatty acids and non-polar chemical compounds in Plumbago zeylanica leaves. To identify fatty acids in saponifiable matter further esterification was performed. All the fatty acid methyl esters and non-polar chemical compounds were identified using NIST library data. 8 fatty acid methyl esters and 6 non-polar chemical compounds were identified. We have analyzed fatty acids and non-polar chemical compounds by the same GC-MS method. Conclusions: The present analysis showed that Plumbago Zeylanica leaves contain 8 fatty acids and 6 non-polar chemical compounds. Principal determination of the research was development of efficient method to identify non-polar compound from plant by single injection using chromatographic technique

Objectives

Methods

Results

Conclusion