Abstract
Background & Objective:Dengue infection is an arthropod borne disease caused by Dengue virus in humans. Dengue virus infection has more potential to produce severe form of the disease with more severe symptoms. Proper diagnosis of dengue fever is very important for its safe management. The objective of this study was to evaluate the non structural protein-1 (NS1) positive parameter for identification of dengue fever by using ELISA from 2013 dengue outbreak in Khyber Pakhtunkhwa.Methods:It was a cross sectional study conducted among 384 patients tested for dengue admitted to different hospitals of Khyber Pakhtunkhwa April to December 2013 with symptoms related to classical dengue fever. Written informed consent was taken from 100 NS1 positive diagnosed patients, and 3 to 5 ml blood sample was collected for confirmation through ELISA testing. ELISA test for dengue IgG and IgM was performed two time in order to confirm the dengue cases. Data was entered and analyzed by using SPSS version 16.Result:The study performed on 100 NS1 positive samples of patients, admitted to hospitals with symptoms related to classical dengue fever, indicated that after performing the IgM and IgG capture ELISA test only 76 samples were actually found positive for dengue. The rest of the 24 samples were found negative for both IgM and IgG capture ELISAs. The study also revealed that 90.8 % patients had primary dengue infection and 35.5% patients had secondary dengue infection. Most patients were between the age of 10-20 years (26%), among them19.7% were having primary dengue infection. Among 10-20 years of age 50% female patients were false dengue patients.Conclusion:About 24 % NSI protein positive samples were found negative for both IgM and IgG capture ELISAs showed that NS1protein positivity does not confirm actual dengue infection.
Highlights
Dengue virus (DENV) is a causative agent of Dengue fever
Other viruses belonging to the same genus include Yellow fever virus, Japanese encephalitis virus, St Louis encephalitis virus that are prevalent in Pakistan and which antigenically cross react with Dengue virus.[2]
The samples were assayed for the detection of both IgM and IgG antibodies against antigen of dengue virus separately
Summary
Dengue virus (DENV) is a causative agent of Dengue fever. Dengue virus (DENV) has four antigenically distinct serotypes (DENV-1, DENV2, DENV-3, and DENV-4) all of which are able to cause complete infection.[1]. Dengue virus is a small virus whose genome is comprised of single strand of positive sense RNA (ssRNA) that is approximately 11kb in length This ssRNA genome encodes merely ten proteins. Result: The study performed on 100 NS1 positive samples of patients, admitted to hospitals with symptoms related to classical dengue fever, indicated that after performing the IgM and IgG capture ELISA test only 76 samples were found positive for dengue. The rest of the 24 samples were found negative for both IgM and IgG capture ELISAs. The study revealed that 90.8 % patients had primary dengue infection and 35.5% patients had secondary dengue infection. Conclusion: About 24 % NSI protein positive samples were found negative for both IgM and IgG capture ELISAs showed that NS1protein positivity does not confirm actual dengue infection
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