Evaluation of non-rhizospheric endophytic bacteria for the management of dry root rot of chickpea

Abstract

Chickpea (Cicer arietinum L.) is an important pulse crop in India and chief source of dietary protein in the vegetarian diet. The important Destructive disease in chickpea is dry root rot caused by necrotrophic fungus Rhizoctonia bataticola (Taub.) Butler emerging as a serious threat to the production worldwide. The screening of endophytes of leaf and stem isolates under dual culture used as two media PDA and TSA. The 12 isolates are significant on but three isolates are EBS-2, EBS-3, EBS-4 higher suppression towards on Rhizoctonia bataticola. These three isolates used as further methods that roll towel method (vitro) and net house (Pot Cultivation). The roll towel observations to be recorded as Germination percentage, Root, shoot lengths and vigour index. The EBL-3 and EBL-4 with highest germination percentage, Root length were EBL-2, 3 and shoot length, vigour index of EBL-3 highest percentage. The effect of EB on Rhizoctonia bataticola under pot culture observations were recorded that phenological parameters are Germination percentage, Pre-Post, Total mortality, Shoot, Root lengths and Fresh dry weights with different cohabitation on disease incidence. The cohabitation of T8EBL (2+3+4) T7EBL (4+2) are significant on four genotypes JG14, JG16, JG62 and JG315 with inoculation of pathogen. The EBL-3 isolate is that significant correlates in field and lab conditions.