Abstract
Chitosan (CS) is a natural polymer with applications in wound restorations and coatings for artificial implants. However, the lack of antimicrobial activity restricts its further application. Thus, the purpose of this study is to prepare CS/AuNPs film by adding gold nanoparticles (AuNPs) to modify chitosan. Through transmission electron microscopy, we found that the presence of CS can effectively prevent the agglomeration of nanoparticles. Furthermore, through the CCK experiment, we found that AuNPs at a certain concentration can improve the adhesion and proliferation of cells, which has also been proved in animal experiments, including gross appearance, histological observations, and immunofluorescence staining. Such findings support the feasibility of using the film as a promising candidate for tissue engineering of skin in a near future.
Highlights
Advances in Materials Science and Engineering clinical practice [11, 12]
Chitosan can only play an antibacterial role in the acidic medium, since it possesses poor solubility at high pH; in addition, the PH value of the film should be neutral in the preparation process. erefore, interesting phenomena can be found in many studies that chitosan solution has antibacterial property while chitosan film does not [13]
CS/AuNP film was synthesized by physical blends of CS with AuNPs, which was characterized with transmission electron microscope (TEM)
Summary
Despite the specific mechanism of this antimicrobial activity not being yet fully understood, several generally accepted hypothesis include surface charge, inhibition of mRNA, and blocking the oxygen path This antibacterial activity is limited by a number of factors, especially environmental PH. With the small size and large surface area, nanoparticles can be desirable delivery vehicles They carry intrinsic properties beneficial for wound healing, including excellent antioxidant and antimicrobial activities as well as anti-inflammatory and antiangiogenic activities [15, 16]. For a direct contact-killing mechanism, AuNPs adhere to the surface of bacterial cells by electrostatic interaction in the form of metallic gold which causes impaired cell membrane integrity, leakage of cell contents, and production of reactive oxygen species (ROS) [20]. The use of CS for stabilizing gold nanoparticles has gained attention recently, preventing AuNPs from agglomeration
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