Evaluation of near-infrared Raman spectroscopy in the differentiation of cortical bone, trabecular bone, and Bio-Oss bone graft: an ex-vivo study

Abstract

We evaluated the ability of near-infrared Raman spectroscopy (near-IR RS) to differentiate among cortical bone, trabecular bone, and Bio-Oss, a bovinebone-based graft material. We obtained a thinly sliced section of the mandible to collect cortical and trabecular bone samples and placed compacted Bio-Oss bone graft into a partially edentulous mandible in a dry human skull to obtain a comparable Bio-Oss sample. We performed near-IR RS of the 3 samples and evaluated the resultant Raman spectra to evaluate their differences. We identified 3 sets of spectroscopic markers that differentiated Bio-Oss from human bone. The first consisted of significant shifts in the location of the 960 cm-1 phosphate (PO43-) peak and a reduction in its width, suggesting that Bio-Oss is more crystalline than bone. The second was the reduced carbonate content of Bio-Oss compared to bone, as determined from the 1070 cm-1/960 cm-1 peak area ratio. The final marker was the lack of collagen-associated peaks in Bio-Oss compared to cortical and trabecular bone. Near-IR RS can reliably differentiate human cortical and trabecular bone from Bio-Oss via 3 sets of spectral markers associated with mineral crystallinity, carbonate content, and collagen content that differ significantly between them. Integrating this modality into dental practice may assist in implant treatment planning.