Abstract
Potato tuber moth, Phthorimaea operculella Zeller, is a highly destructive pest of cultivated potato, Solanum tuberosum L., and is responsible for damage to both leaf and tuber tissues. Host plant resistance is a central component to developing an integrated pest management program to control potato tuber moth. This research tested the efficacy of a codon-modified CryV-Bacilius thuringiensis (CryV-Bt) gene constitutively expressed in potato and the combined effect of CryV-Bt expression with natural host plant resistance mechanisms in potato. ‘Lemhi Russet’ and 2 lines with host plant resistance mechanisms, USDA8380-1 (leaf leptines) and L235-4 (glandular trichomes), along with the CryV-Bt-transgenic lines of each of these 3 genotypes were examined. Detached leaf bioassays were conducted to examine control of potato tuber moth. Nontransformed Lemhi Russet and L235-4 were susceptible to potato tuber moth, while 54%potato tuber moth mortality was found when first instar larvae fed on USDA8380-1Ieaves. High levels of expression occurred in the CryV-Bt transgenic lines, with up to 96% potato tuber moth mortality. These transgenic lines provide a germplasm base to examine combined insect-resistance mechanisms as a means to achieve durable host plant resistance.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.