Abstract

In recent years, one has been exposed to a number of advances in the methods of human identity analysis. Why, in this high-tech age, one may wonder, should time be devoted to another paper on polyacrylamide gel electrophoresis (PAGE)? The answer to this question is intimately associated with the increasing popularity of short tandem repeat (STR) analysis. Many laboratories are using PAGE to both optimize PCR conditions and to obtain databases, and there are almost as many PAGE systems being used as there are loci being analyzed! It is time to standardize the PAGE conditions, so that inter-laboratory variation in results can be minimized. In this communication, three PAGE systems are compared, in order to generate a discussion that may lead to a consensus towards the adoption of a single PAGE system for STR analysis.

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